Journal: American Journal of Clinical and Experimental Urology

Article Title: Predictive value of pseudouridine in prostate cancer

doi:

Figure Lengend Snippet: A. Representative images (10× with 40× insert) of normal adjacent tissues (NAT) and adenocarcinomas. A tissue micro array (TMA) purchased from US Biomax, Inc. (PR242b) was stained for pseudouridine and imaged at 10X and 40X. Cores with Gleason 3+3, 4+3 and 5+4 were used for representative images. Staining is primarily localized to the cytoplasm of glandular cells with negligible staining of the gland cells belonging to normal adjacent tissues (NATs). B. Plot of H-scores of normal adjacent tissues and adenocarcinomas. Each individual core was evaluated by an in-house pathologist for the presence of cancer, and given an H-score. The final H-score is the summation of (1+i)pi where i is the intensity score [0 (lowest)1+, 2+, 3+, 4+(highest)] and pi is the percent of the cells with that intensity. The p-value was calculated using a two-tailed unpaired t-test in Prism, where an alpha value of 0.05 was considered significant.

Article Snippet: TMA immunohistochemistry and analysis Human prostate cancer tissue microarray (TMA) PR242b was purchased from US Biomax, Inc. (Derwood, MD, USA).

Techniques: Microarray, Staining, Two Tailed Test

Journal: American Journal of Clinical and Experimental Urology

Article Title: Predictive value of pseudouridine in prostate cancer

doi:

Figure Lengend Snippet: A. Representative images (10× with 40× insert) of normal adjacent tissues (NAT) and adenocarcinomas. A tissue micro array (TMA) purchased from US Biomax, Inc. (PR242b) was stained for pseudouridine and imaged at 10X and 40X. Cores with Gleason 3+3, 4+3 and 5+4 were used for representative images. Staining is primarily localized to the cytoplasm of glandular cells with negligible staining of the gland cells belonging to normal adjacent tissues (NATs). B. Plot of H-scores of normal adjacent tissues and adenocarcinomas. Each individual core was evaluated by an in-house pathologist for the presence of cancer, and given an H-score. The final H-score is the summation of (1+i)pi where i is the intensity score [0 (lowest)1+, 2+, 3+, 4+(highest)] and pi is the percent of the cells with that intensity. The p-value was calculated using a two-tailed unpaired t-test in Prism, where an alpha value of 0.05 was considered significant.

Article Snippet: Human prostate cancer tissue microarray (TMA) PR242b was purchased from US Biomax, Inc. (Derwood, MD, USA).

Techniques: Microarray, Staining, Two Tailed Test